Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of <i>para</i>-aryl-substituted <i>N</i>-phenyl groups on the photophysical properties of highly fluorescent dibenzo[<i>c</i>,<i>g</i>]carbazole-based chromophores.

RSC advances·2026
Same author

Disruption Dynamics and Charge Transfer of a Single Attoliter Emulsion Droplet Revealed by Combined Fast-Scan Sinusoidal Voltammetry and Short Time Fourier Transform Analysis.

Analytical chemistry·2024
Same author

Electrochemical Characterization of Neurotransmitters in a Single Submicron Droplet.

Biosensors·2024
Same author

Splicing signature database development to delineate cancer pathways using literature mining and transcriptome machine learning.

Computational and structural biotechnology journal·2023
Same author

Baseline metabolic tumour burden improves risk stratification in Hodgkin lymphoma: A Children's Oncology Group study.

British journal of haematology·2023
Same author

Can cartilage intermediate layer protein 1 (CILP1) use as a novel biomarker for canine myxomatous mitral valve degeneration levels or not?

BMC veterinary research·2023
Same journal

<i>In Vitro</i> Skin-Protective Effects of Sumyong Nature, a Kimchi- and Soybean-Derived Fusion-Fermented Microbial Supernatant, in Human Keratinocytes: Involvement of MAPK-Related Signaling.

Journal of microbiology and biotechnology·2026
Same journal

Molecular Cloning, Biochemical Characterization, and Structural Insights into a Flavonoid-Associated Class II 4-Coumarate:CoA Ligase from <i>Sageretia thea</i>.

Journal of microbiology and biotechnology·2026
Same journal

Qingxuan Zhike Granules Modulate Gut Dysbiosis and Enhance Intestinal Repair in Murine <i>Mycoplasma pneumonia</i>.

Journal of microbiology and biotechnology·2026
Same journal

Phenotypic and Genomic Analysis of <i>Pediococcus acidilactici</i> MBEL10321 with Potential Probiotic Properties.

Journal of microbiology and biotechnology·2026
Same journal

CRISPR/Cas12a Technology Combined with Immunochromatographic Strips for the Portable Detection of SFTS Bunyavirus.

Journal of microbiology and biotechnology·2026
Same journal

Bioactive Lipid Mediator Resolvin D5 Suppresses Melanogenesis in α-MSH-Stimulated B16F10 Cells via ROS-Associated p38/β-Catenin/MITF Regulation.

Journal of microbiology and biotechnology·2026
See all related articles

Related Experiment Video

Updated: Jun 15, 2026

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
10:01

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

Highly sensitive PNA array platform technology for single nucleotide mismatch discrimination.

Jae-Jin Choi1, Minjeong Jang, Jihyun Kim

  • 1Panagene Inc., Daejeon 305-510, Korea.

Journal of Microbiology and Biotechnology
|March 9, 2010
PubMed
Summary
This summary is machine-generated.

Peptide nucleic acid (PNA) arrays reliably detect single nucleotide mismatches with superior specificity and sensitivity compared to DNA arrays. These PNA arrays demonstrate excellent stability, maintaining performance after long-term storage.

More Related Videos

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
09:30

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

Related Experiment Videos

Last Updated: Jun 15, 2026

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
10:01

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
09:30

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genetics

Background:

  • Accurate detection of single nucleotide mismatches is crucial for genetic analysis and diagnostics.
  • Traditional DNA arrays face limitations in specificity and sensitivity for distinguishing subtle genetic variations.

Purpose of the Study:

  • To evaluate the performance of peptide nucleic acid (PNA) arrays for discriminating single nucleotide mismatches.
  • To compare the specificity, sensitivity, and stability of PNA arrays against conventional DNA arrays.

Main Methods:

  • Development of a novel PNA probe immobilization technique and optimized hybridization conditions for PNA arrays.
  • Comparative analysis of PNA and DNA arrays using single nucleotide mismatch discrimination assays.
  • Assessment of array stability through long-term storage at room temperature.

Main Results:

  • PNA arrays exhibited significantly higher perfect match-to-mismatch signal ratios and enhanced sensitivity compared to DNA arrays.
  • PNA arrays demonstrated 2.0 to 37.3 times greater specificity and approximately 10 times higher sensitivity than DNA arrays.
  • Mismatch signal intensities on PNA probes ranged from 1.6% to 12.1% relative to perfect match signals.
  • A PNA array maintained its specificity and sensitivity after 12 months of storage at room temperature.

Conclusions:

  • PNA arrays offer a robust platform for highly specific and sensitive detection of single nucleotide mismatches.
  • The developed PNA probe immobilization and hybridization methods contribute to the superior performance of PNA arrays.
  • PNA arrays present a stable and reliable alternative to DNA arrays for genetic variation analysis, with potential for long-term diagnostic applications.