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Published on: July 8, 2020
Bioactive Lipid Mediator Resolvin D5 Suppresses Melanogenesis in α-MSH-Stimulated B16F10 Cells via ROS-Associated
Hyeok Jin Choi1, So Jung Park1, Jeong Won Choi1
1Department of Forest Science, Gyeongkuk National University, Andong 36729, Republic of Korea.
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Excessive melanin production contributes to hyperpigmentation disorders, highlighting the need for safer, mechanism-based depigmenting agents. This study investigated the anti-melanogenic effects of Resolvin D5 (RVD5) in α-MSH-stimulated B16F10 melanoma cells. RVD5 significantly reduced extracellular and intracellular melanin levels without compromising cell viability or cell number. Mechanistically, RVD5 suppressed tyrosinase expression and activity, accompanied by downregulation of MITF and β-catenin. RVD5 also dose-dependently inhibited α-MSH-induced phosphorylation of ERK1/2, p38, and JNK, while pharmacological inhibitor analysis suggested that p38 is a functionally dominant MAPK branch contributing to melanin output. In parallel, RVD5 reduced intracellular ROS levels, and the ROS scavenger NAC mimicked several effects of RVD5, supporting the involvement of ROS-associated signaling. Overall, RVD5 attenuates melanogenesis through antioxidant action, broad MAPK attenuation, and downregulation of the β-catenin/MITF/tyrosinase regulatory pathway.
