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Updated: Jun 15, 2026

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography
Published on: October 17, 2018
High-resolution tomographic diffractive microscopy of biological samples
Bertrand Simon1, Matthieu Debailleul, Anne Beghin
1Laboratoire MIPS-EA2332, Université de Haute-Alsace, IUT Mulhouse, Mulhouse, France.
Abstract:
The authors have developed a tomographic diffractive microscope that combines microholography with illumination from an angular synthetic aperture. It images specimens relative to their complex index of refraction distribution (index and absorption) and permits imaging of unlabelled specimens, with high lateral resolution. The authors now study its use for biological applications, and imaged several preparations with fluorescence confocal microscopy and tomographic diffractive microscopy. The results highlight some interesting features of this instrument, which should attract the interest of biologists for this new technique.
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