Related Experiment Video
Updated: Jun 15, 2026
13:26
Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
Published on: April 17, 2015
Recent advances in DNA sequencing methods - general principles of sample preparation
1Laboratory for Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Scheeles väg 1, SE-171 77 Stockholm, Sweden. sten.linnarsson@ki.se
Experimental Cell Research
|March 10, 2010
Summary
High-throughput DNA sequencing has advanced rapidly, but preparing genomic and RNA samples for analysis remains a significant bottleneck. This review covers current methods for sample preparation in next-generation sequencing.
Area of Science:
- Genomics
- Molecular Biology
- Biotechnology
Background:
- DNA sequencing technologies have undergone rapid advancements over the past three decades.
- The human genome project demonstrated the potential of large-scale DNA sequencing.
- Next-generation sequencing instruments offer unprecedented data output capabilities.
Purpose of the Study:
- To review the current state-of-the-art in sample preparation for high-throughput sequencing.
- To identify challenges and advancements in preparing genomic and RNA samples.
- To provide an overview for researchers utilizing next-generation sequencing.
Main Methods:
- Review of current literature and technologies in nucleic acid sample preparation.
- Analysis of automation and throughput in sample processing workflows.
- Comparison of methods for genomic DNA and RNA sample preparation.
Main Results:
- Next-generation sequencing instruments are highly automated, but upstream sample processing is a major challenge.
- Significant progress has been made in preparing genomic samples, but RNA sample preparation requires further optimization.
- Various techniques exist for library preparation, but efficiency and standardization vary.
Conclusions:
- Optimizing upstream sample processing is crucial for maximizing the utility of high-throughput sequencing.
- Further innovation in sample preparation methods is needed to fully realize the potential of next-generation sequencing.
- Standardized and automated sample preparation protocols will enhance reproducibility and accessibility in genomic research.
More Related Videos
Related Concept Videos
01:57Sanger Sequencing
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
03:21RNA-seq
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
03:00Next-generation Sequencing
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
01:24DNA Isolation
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
01:05Maxam-Gilbert Sequencing
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
Challenges of the Maxam-Gilbert Method
The...