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Updated: Jun 5, 2025

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Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
Published on: August 25, 2020
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Applying single-cell and single-nucleus genomics to studies of cellular heterogeneity and cell fate transitions in
Igor Adameyko1,2, Trygve Bakken3, Aparna Bhaduri4
1Department of Neuroimmunology, Center for Brain Research, Medical University of Vienna, Vienna, Austria.
Nature Neuroscience
|December 3, 2024
Abstract:
Single-cell and single-nucleus genomic approaches can provide unbiased and multimodal insights. Here, we discuss what constitutes a molecular cell atlas and how to leverage single-cell omics data to generate hypotheses and gain insights into cell transitions in development and disease of the nervous system. We share points of reflection on what to consider during study design and implementation as well as limitations and pitfalls.

