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Updated: Jun 15, 2026

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
Published on: September 11, 2016
Epidemiologic features and virus isolation of enteroviruses in Northern Taiwan during 2000-2008
Kuo-Chien Tsao1, Chung-Guei Huang, Ya-Ling Huang
1Research Center for Emerging Viral Infections, Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan.
Abstract:
The susceptibility of five cell lines, RD, MRC-5, MK-2, Hep-2 and A549, for various serotypes of human enteroviruses was evaluated. RD cells were susceptible to most serotypes of enteroviruses, especially for human enterovirus A. Consequently, a high prevalence of human enterovirus A in Taiwan may reflect the relative importance of RD cells in the clinical virology laboratory. However, susceptibilities of RD cells to coxsackievirus A9 and A24, and echovirus 9 and 11 were exceptionally low. Alternatively, MRC-5 or MK-2 cells can improve the isolation of these four enteroviruses.

