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Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Design and Fabrication of an Optical Fiber Made of Water
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Frustrated-total-internal-reflection multimode fiber-optic hydrophone.

W B Spillman, D H McMahon

    Applied Optics
    |March 11, 2010
    PubMed
    Summary
    This summary is machine-generated.

    A novel multimode fiber-optic hydrophone utilizing frustrated total internal reflection was developed. This acoustic sensor demonstrates high sensitivity, detecting minimal pressure and static displacements, and is compatible with existing fiber-optic systems.

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    Area of Science:

    • Optoelectronics
    • Acoustic Sensing
    • Fiber Optics

    Background:

    • Fiber-optic hydrophones offer advantages in harsh environments.
    • Frustrated total internal reflection (FTIR) is a viable principle for optical sensing.

    Purpose of the Study:

    • To construct and evaluate a multimode fiber-optic hydrophone.
    • To assess the hydrophone's sensitivity to acoustic waves and static displacements.
    • To determine the minimum detectable pressure and displacement.

    Main Methods:

    • Fabrication of a multimode fiber-optic hydrophone based on FTIR.
    • Testing the device's response to acoustic waves in water.
    • Measuring sensitivity to applied static displacements in air.
    • Comparing experimental results with theoretical predictions.

    Main Results:

    • The hydrophone's sensitivity to acoustic waves aligns with predictions from static displacement tests.
    • The minimum detectable pressure was measured at 62 dB re 1 µPa at 500 Hz.
    • The sensor can detect static displacements as small as 4.8 x 10⁻³ Å.
    • The device exhibits compatibility with standard multimode fiber-optic components.

    Conclusions:

    • The developed multimode fiber-optic hydrophone is a sensitive acoustic sensor.
    • The FTIR principle is effective for fiber-optic hydrophone design.
    • The sensor's performance is well-characterized and suitable for practical applications.