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Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

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Related Experiment Video

Updated: Jun 15, 2026

Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo
06:37

Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo

Published on: September 18, 2019

Preventing tissue injury using siRNA.

Zhu-Xu Zhang1, Marianne E Beduhn, Xiufen Zheng

  • 1Department of Surgery, University of Western Ontario, London, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2010
PubMed
Summary
This summary is machine-generated.

RNA interference (RNAi) offers potent gene silencing, overcoming limitations of traditional therapies. This technology shows promise for treating diseases and advancing clinical therapeutics, particularly in tissue injury and transplantation.

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Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages
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Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

Published on: November 3, 2014

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Last Updated: Jun 15, 2026

Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo
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Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo

Published on: September 18, 2019

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages
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Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

Published on: November 3, 2014

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • RNA interference (RNAi) is a natural cellular process for gene silencing.
  • Small interfering RNA (siRNA) therapeutics offer a novel approach to gene silencing.
  • RNAi overcomes limitations of traditional therapeutics like antibodies and small molecules.

Purpose of the Study:

  • To review the applications of RNAi in tissue injury.
  • To explore the potential of RNAi in transplantation.

Main Methods:

  • Review of existing literature on RNAi applications.
  • Analysis of siRNA delivery and efficiency challenges.
  • Discussion of RNAi's therapeutic potential in specific contexts.

Main Results:

  • RNAi demonstrates effective gene silencing for therapeutic benefit.
  • Successful applications in treating hepatitis, viral infections, and cancer.
  • RNAi presents a promising therapeutic strategy for tissue injury and transplantation.

Conclusions:

  • RNAi is a powerful tool for selective gene silencing.
  • Advancements in siRNA delivery and efficiency are crucial for clinical translation.
  • RNAi holds significant potential to revolutionize clinical therapeutics, especially in tissue injury and transplantation.