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Updated: Jun 15, 2026

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Surface plasmon resonance: a general introduction.

Nico J de Mol¹, Marcel J E Fischer

¹Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)

|March 11, 2010

Summary

This summary is machine-generated.

Surface Plasmon Resonance (SPR) analysis offers unique insights into biomolecular interactions by quantifying binding affinity and kinetics. This guide explains SPR principles, addressing mass transport limitations and data conversion for diverse applications.

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Published on: July 21, 2018

Area of Science:

Biophysical Chemistry
Analytical Chemistry

Background:

Surface Plasmon Resonance (SPR) analysis is a powerful technique for studying biomolecular interactions, providing quantitative data on binding constants (affinity) and kinetics.
Understanding the physical principles of SPR, including the evanescent field's depth, is crucial for interpreting experimental results and recognizing method limitations.

Discussion:

The impact of kinetics on biomolecular interactions is highlighted, emphasizing the importance of kinetic analysis in SPR studies.
Mass transport limitation (MTL) is a common challenge in kinetic analysis; a practical model is presented to estimate and mitigate MTL through experimental design.

Key Insights:

SPR enables the determination of binding affinity and kinetic parameters for molecular interactions.
A model for estimating Mass Transport Limitation (MTL) helps optimize experimental design to ensure accurate kinetic data.
Methods for converting SPR signals (Response Units or millidegrees) into mass per surface unit are provided.

Outlook:

The chapter concludes with an overview of available commercial SPR instrumentation.
This foundational knowledge facilitates the rational application and interpretation of SPR data across various scientific disciplines.