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Updated: Jun 15, 2026

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Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
Published on: January 22, 2014
High-affinity immobilization of proteins using biotin- and GST-based coupling strategies
Stephanie Q Hutsell1, Randall J Kimple, David P Siderovski
1Department of Biochemistry and Biophysics, The University of North Carolina, Chapel Hill, NC, USA.
Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2010
Abstract
None:
Surface plasmon resonance (SPR) is a highly sensitive method for the detection of molecular interactions. One interacting partner is immobilized on the sensor chip surface while the other is injected across the sensor surface. This chapter focuses on high-affinity immobilization of protein substrates for affinity and kinetic analyses using biotin/streptavidin interaction and GST/anti-GST-antibody interaction.

