Comparative histopathological effects of ethylene-bis-dithiocarbamate on the testis in rabbits and rats

Maneb is an ethylene-bis-dithiocarbamate fungicide used in the control of the fungal diseases of plants. Males of domestic rabbits Oryctolagus cuniculus and Wistar rats received daily by gavage 2 and 3 mg/kg body weight of Maneb for 3 weeks. At the end of the treatment, the animals were sacrificed and the testis's samples were weighed and placed in formol 10% solution. The histopathology changes study was made in accordance with Martoja and Martoja, 1967 method. The obtained results show a very considerable reduction of the weight of testes in treated animals compared to the control group especially in the rabbits. A very reduction in testosterone concentration was noticed in the treated rabbits. The histological observation showed that no effect was observed in the testes in rats. Then, the testicle of treated rabbits with 2 mg/kg body weight reveal seminal tubules in involution Sertolienne and Leydig cells are a little too visible. White rabbits treated 3 mg/kg indicates that there is no spermatozoa in the epididymis, the most of the tubules are empty as compared to control. The testis in the control show normal spermatogenesis and the mitoses are distributed to all levels and spermatozoa are observed in many tubules and epididymis. In conclusion, administration Maneb with the used doses affects histological deformation in the testis of rabbits.