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Related Concept Videos

RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

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Updated: Jun 15, 2026

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae
09:12

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

Published on: February 27, 2026

Sequence and structure analysis of noncoding RNAs.

Stefan Washietl1

  • 1Department of Theoretical Chemistry, University of Vienna, Vienna, Wien, Austria.

Methods in Molecular Biology (Clifton, N.J.)
|March 12, 2010
PubMed
Summary
This summary is machine-generated.

This study overviews computational methods for analyzing noncoding RNAs (ncRNAs), aiding in understanding their cellular functions. Key techniques cover structure prediction, homology searching, and identifying novel ncRNAs and their interactions.

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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Last Updated: Jun 15, 2026

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae
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Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

Published on: February 27, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

Area of Science:

  • Bioinformatics
  • Molecular Biology
  • Genomics

Background:

  • Noncoding RNAs (ncRNAs) are crucial functional molecules in cellular processes.
  • Understanding ncRNA function requires sophisticated analytical approaches.
  • Computational methods provide powerful tools for ncRNA research.

Purpose of the Study:

  • To provide a concise overview of essential computational techniques for analyzing ncRNAs.
  • To facilitate a deeper understanding of ncRNA functions through computational analysis.
  • To cover a range of methods applicable to diverse ncRNA types.

Main Methods:

  • Secondary structure prediction from primary sequences.
  • Consensus structure prediction for homologous ncRNA sequences.
  • Database searching using sequence and structure comparisons.
  • Annotation of specific ncRNA classes (tRNA, rRNA, snoRNA, miRNA).
  • De novo prediction of novel ncRNAs.
  • Prediction of RNA/RNA interactions, including miRNA target prediction.

Main Results:

  • The study outlines a comprehensive suite of computational tools for ncRNA analysis.
  • These methods enable detailed investigation from sequence to interaction level.
  • The presented techniques are applicable to various ncRNA classes and functions.

Conclusions:

  • Computational approaches are indispensable for deciphering ncRNA roles.
  • A robust understanding of ncRNA function relies on integrated computational strategies.
  • This overview serves as a foundational guide for researchers in the field.