Related Experiment Video
Updated: Jun 15, 2026

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
[MEKC-DAD fingerprint of Radix et Rhizoma Rhei]
Xunhong Liu1, Junsong Li, Yuechan Zhang
1Pharmacology College, Nanjing University of Chinese Medicine, Nangjing 210046, China. liuxunh1959@sohu.com
Objective:
To establish the analytical method for the fingerprint of Radix et Rhizoma Rhei by MEKC-DAD and compare the fingerprints of Radix et Rhizoma Rhei and its processed products.
Method:
Based on the mode of micellar electrokinetic chromatography, 25 mmol x L(-1) borax -25 mmol x L(-1) SDS-10% acetonitrile was selected for the running buffer (pH 9.2). The separation voltage was 12 kV and the detection wavelength was set at 254 nm. Rhein was used as a reference standard, the chromatographic fingerprint was determined via the data analyzed by fuzzy cluster and fingerprint similarity evaluation software to compare the similarity of samples.
Result:
MEKC-DAD fingerprints with 11 common peaks of 10 batches of Radix et Rhizoma Rhei from the place of the genuine were established preliminarily. It was discovered that a small number of samples differed from others. Regarding to the fingerprints of Radix et Rhizoma Rhei and its processed products, there were obvious differences in the relative areas of common peaks.
Conclusion:
The method is reliable, accurate and can be used for quality control of Radix et Rhizoma Rhei.
Related Concept Videos
IR Frequency Region: Fingerprint Region
The...
Radicals