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Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)
Published on: April 20, 2015
Selective perborate signaling by deprotection of fluorescein and resorufin acetates
Myung Gil Choi1, Sunyoung Cha, Ji Eun Park
1Department of Chemistry, Chung-Ang University, Seoul 156-756, Korea.
Abstract:
The acetate derivatives of fluorescein and resorufin exhibited a prominent turn-on type signaling behavior toward BO(3)(-) ions over other common anions. Signaling is based on the selective deprotection of acetate groups by perborate, which resulted in significant chromogenic and fluorogenic signaling. Compound 1 also exhibited a pronounced perborate selectivity over other commonly used oxidants in 90% aqueous acetonitrile solution.
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