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Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: Jun 15, 2026

Optogenetics Identification of a Neuronal Type with a Glass Optrode in Awake Mice
07:51

Optogenetics Identification of a Neuronal Type with a Glass Optrode in Awake Mice

Published on: June 28, 2018

Fluorescent pipettes for optically targeted patch-clamp recordings.

Daisuke Ishikawa1, Naoya Takahashi, Takuya Sasaki

  • 1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.

Neural Networks : the Official Journal of the International Neural Network Society
|March 13, 2010
PubMed
Summary
This summary is machine-generated.

This study presents a simpler method for targeting neurons using fluorophore-coated pipettes. This technique allows simultaneous imaging of neurons and pipette tips, improving accuracy in patch-clamp recordings.

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Related Experiment Videos

Last Updated: Jun 15, 2026

Optogenetics Identification of a Neuronal Type with a Glass Optrode in Awake Mice
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Published on: June 28, 2018

One-channel Cell-attached Patch-clamp Recording
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Pressure-polishing Pipettes for Improved Patch-clamp Recording

Published on: October 22, 2008

Area of Science:

  • Neuroscience
  • Biophysics
  • Cell Biology

Background:

  • Targeted patch-clamp recording is crucial for studying individual neuron physiology within complex networks.
  • Current methods rely on sequential fluorescence and transmitted light microscopy, which can be cumbersome and prone to optical complications.
  • Accurate neuron identification and approach are essential for reliable electrophysiological measurements.

Purpose of the Study:

  • To develop a simplified and more efficient method for targeting specific neurons during patch-clamp recordings.
  • To overcome limitations associated with traditional neuron visualization and pipette manipulation techniques.
  • To enhance the accessibility and reliability of in situ electrophysiological studies.

Main Methods:

  • Development of novel fluorophore-coated patch-clamp pipettes.
  • Simultaneous imaging of fluorescently labeled neurons and pipette tips at the same fluorescence wavelength.
  • Integration of imaging and micromanipulation within a single microscope field.
  • Assessment of the impact of fluorophore coating on pipette and neuronal electrical properties.

Main Results:

  • The fluorophore-coated pipette method allows simultaneous visualization of target neurons and pipette tips.
  • This technique eliminates the need for switching between microscopy modes, simplifying the targeting process.
  • No adverse effects of the fluorophore coating were observed on the electrical properties of pipettes or neurons.
  • The method proved effective for precise targeting of neurons and their neurites.

Conclusions:

  • Fluorophore-coated pipettes offer a simpler, more efficient, and robust approach to neuron targeting in patch-clamp recordings.
  • This technique minimizes optical complications and mechanical difficulties, making it widely applicable.
  • The method enhances the feasibility of in situ electrophysiological studies by improving pipette micromanipulation under online visual control.