Proteome analysis for rat saliva
Kiyotoshi Inenaga1, Naoyuki Yamada, Reiko Yuji
1Division of Physiology, Department of Biosciences, Kyushu Dental College, Kitakyushu, Japan.
Insights
This study presents a new proteomic method for analyzing saliva, enabling the detection of low-concentration disease biomarkers. This approach improves biomarker discovery, especially for vulnerable populations like children and the elderly.
Area of Science:
- Biochemistry
- Proteomics
- Biomarker Discovery
Background:
- Proteome analysis aids in discovering disease biomarkers.
- Saliva is a non-invasive biofluid, ideal for diagnostics, particularly in children and the elderly.
- High protein abundance in saliva can obscure low-concentration biomarker detection.
Purpose of the Study:
- To develop an advanced proteomic method for sensitive biomarker detection in saliva.
- To overcome challenges in identifying low-abundance proteins within complex saliva samples.
Main Methods:
- Development of a novel shotgun proteomic technique.
- Utilized two-dimensional nano-flow liquid chromatography coupled with tandem mass spectrometry (2D nano-LC-MS/MS).
Main Results:
- The developed method successfully detects proteins quantitatively.
- Effective identification of low-concentration proteins in small saliva volumes was achieved.
- The method addresses the issue of high protein abundance obscuring biomarker signals.
Conclusions:
- The novel 2D nano-LC-MS/MS proteomic method enhances saliva biomarker discovery.
- This technique offers a sensitive and quantitative approach for analyzing saliva proteomes.
- The method holds promise for improved disease prevention and diagnosis, especially in pediatric and geriatric populations.
Abstract:
Proteome analysis is a popular method to discover biomarkers for the prevention and diagnosis of diseases. Since saliva is a non-invasively available body fluid, gathering of saliva causes minimal harm to patients. Therefore, detection of proteins for the prevention and diagnosis from the saliva sample may be the preferred method, especially for children and elderly people. However, the abundance of salivary proteins and contaminant proteins from food and mouth bacteria obscure identification of proteins present in the saliva at low concentrations. To address this problem, we developed a shotgun proteomic method using two-dimensional nano-flow LC tandem mass spectrometry. We report here that our method is able to detect proteins quantitatively even in small sample volumes of saliva.
