Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 15, 2026

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage
09:53

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Published on: February 7, 2021

Single-molecule studies of RecBCD.

Thomas T Perkins1, Hung-Wen Li

  • 1MCD Biology, JILA, NIST & CU, University of Colorado at Boulder, Boulder, CO, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 13, 2010
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evolution of N-terminal mechanical lability as a determinant for Type III secretion.

Protein science : a publication of the Protein Society·2026
Same author

Force-Induced Ankle Opening Reveals Mechanical Stabilization of the Ankle of Human β-Cardiac Myosin.

ACS nano·2026
Same author

A Type III secretion system effector evolved to be mechanically labile and initiate unfolding from the N-terminus.

bioRxiv : the preprint server for biology·2026
Same author

SWI5-SFR1 reduces RAD51 recombinase extending units during filament assembly.

Nucleic acids research·2025
Same author

Mug20-Rec25-Rec27 binds DNA and enhances meiotic DNA break formation via phase-separated condensates.

Nucleic acids research·2025
Same author

Hop2-Mnd1 functions as a DNA sequence fidelity switch in Dmc1-mediated DNA recombination.

Nature communications·2024

The RecBCD enzyme

Area of Science:

  • Molecular biology
  • Biophysics

Background:

  • RecBCD is a molecular motor essential for DNA repair.
  • Understanding its DNA translocation is key to its function.

Purpose of the Study:

  • To characterize the translocation properties of RecBCD using high-resolution single-molecule techniques.
  • To investigate the motor activity and catalytic rate of RecBCD.

Main Methods:

  • Developed a high-spatial resolution optical-trapping assay for RecBCD.
  • Anchored RecBCD to a surface via genetically engineered biotin.
  • Measured DNA translocation and characterized motor properties.

Main Results:

  • RecBCD exhibits continuous motion with a step size below 6 base pairs.

More Related Videos

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
10:11

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

Published on: July 26, 2024

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

Related Experiment Videos

Last Updated: Jun 15, 2026

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage
09:53

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Published on: February 7, 2021

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
10:11

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

Published on: July 26, 2024

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

  • Observed abrupt and sustained changes in the enzyme's catalytic rate.
  • Validated RecBCD activity using biochemical and tethered particle motion assays.

    • Conclusions:

    • The developed optical-trapping assay provides high-resolution insights into RecBCD motor activity.
    • RecBCD displays complex translocation dynamics, including persistent changes in catalytic rate.
    • Further studies can elucidate the mechanisms behind these observed dynamic behaviors.