Jove
Visualize
Contact Us

Related Concept Videos

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.9K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.9K
Homologous Recombination02:31

Homologous Recombination

52.3K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
52.3K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.1K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.1K
Generation of Straight or Branched Actin Filaments01:14

Generation of Straight or Branched Actin Filaments

3.0K
The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
3.0K
Formation of Intermediate Filaments00:57

Formation of Intermediate Filaments

3.2K
Intermediate filaments are cytoskeletal proteins with higher tensile strength and flexibility than microfilaments and microtubules. Unlike the other two cytoskeletal proteins, intermediate filament formation lacks the enzymatic activity to hydrolyze nucleotides like ATP and GTP to generate energy for polymerization. Therefore, the formation of intermediate filaments is multistep self-assembly. The involvement of any accessory proteins in intermediate filament formation has not yet been...
3.2K
Disassembly of Intermediate Filaments01:35

Disassembly of Intermediate Filaments

2.1K
Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A phosphorylation switch at MRE11 links ATM-ATR and calcium signaling to safeguard stalled replication fork stability.

Research square·2026
Same author

The DNA helicase HELQ promotes replication fork reversal in coordination with BRCA2- and FANCD2-mediated repair pathways.

Nucleic acids research·2026
Same author

Elucidating HLTF-Mediated DNA Fork Remodeling via Native Mass Spectrometry.

Journal of the American Chemical Society·2026
Same author

BMAL2 is a druggable target for ovarian clear cell carcinoma (OCCC).

EMBO molecular medicine·2026
Same author

Slippage reconfiguration of trinucleotide repeat hairpins impedes resolution by human replication protein A.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

RAD51 D-loop structures reveal the mechanism of eukaryotic RAD51-mediated strand exchange.

Nature communications·2025
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Sep 14, 2025

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

8.1K

SWI5-SFR1 reduces RAD51 recombinase extending units during filament assembly.

Yingying Hu1, Yen-Chan Chang1, You-Yang Tsai1

  • 1Department of Chemistry, National Taiwan University, 10617 Taipei, Taiwan.

Nucleic Acids Research
|July 19, 2025
PubMed
Summary
This summary is machine-generated.

Mouse SWI5-SFR1 protein stabilizes RAD51 filaments during DNA repair. This accessory protein promotes uniform filament growth by modulating RAD51

More Related Videos

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

1.9K
Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
11:40

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Published on: June 25, 2013

12.2K

Related Experiment Videos

Last Updated: Sep 14, 2025

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

8.1K
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

1.9K
Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
11:40

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Published on: June 25, 2013

12.2K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Homologous recombination is essential for high-fidelity DNA double-strand break repair.
  • RAD51 filament assembly is a critical, regulated step involving nucleation and extension.
  • Accessory proteins like SWI5-SFR1 modulate RAD51 filament dynamics and recombination efficiency.

Purpose of the Study:

  • To investigate the role of mouse SWI5-SFR1 in the RAD51 filament extension phase.
  • To elucidate the mechanism by which SWI5-SFR1 influences RAD51 assembly and DNA binding.

Main Methods:

  • Step-size analysis of RAD51 filament assembly.
  • Investigated the effect of mouse SWI5-SFR1 on RAD51 dissociation probability and oligomeric state.

Main Results:

  • Mouse SWI5-SFR1 significantly reduces RAD51 dissociation during filament extension, leading to more uniform growth.
  • RAD51 primarily assembles as octamers, reflecting its intrinsic oligomeric nature.
  • SWI5-SFR1 shifts the RAD51 assembly step-size distribution towards tetramers, indicating modulation of oligomeric state.

Conclusions:

  • SWI5-SFR1 facilitates RAD51 filament extension and stabilizes DNA binding by altering RAD51's oligomeric state.
  • Findings bridge the understanding of RAD51 filament nucleation and extension stages.
  • Proposes a comprehensive mechanism for RAD51 filament formation and regulation by accessory proteins for genome stability.