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Updated: Jun 15, 2026
Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
Published on: January 30, 2019
A method to study the role of DDX3 RNA helicase in HIV-1
Chia-Yen Chen1, Venkat R K Yedavalli, Kuan-Teh Jeang
1Molecular Virology Section, Laboratory of Molecular, Microbiology, the NIAID, NIH, Bethesda, MD, USA.
Abstract:
Viral replication requires the use of host cell proteins and enzymes. Many viruses utilize viral helicases at various stages of their life cycle; these viruses have evolved to encode directly helicase or helicase-like proteins. In contrast, the genomes of retroviruses are devoid of viral helicases. Human immunodeficiency virus (HIV-1) has adopted the ability to use one or more cellular RNA helicases for its replicative life cycle. In this chapter, we briefly summarize the approach for assaying the RNA unwinding activity of RNA helicases measuring the effect of helicase inhibitors on HIV-1 replication.
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