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Glucose Homeostasis: Pancreatic Islets and Insulin Secretion01:27

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Proinsulin C-peptide prevents type-1 diabetes-induced decrease of renal Na+-K+-ATPase alpha1-subunit in rats.

Lina Nordquist1, Kohei Shimada, Tatsuya Ishii

  • 1Laboratory of Biochemistry, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.

Diabetes/Metabolism Research and Reviews
|March 13, 2010
PubMed
Summary

C-peptide prevents diabetes-induced reduction in kidney Na(+)/K(+)-ATPase alpha1-subunit expression. This suggests C-peptide plays a role in maintaining kidney function during diabetes.

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Area of Science:

  • Nephrology
  • Endocrinology
  • Molecular Biology

Background:

  • Diabetic nephropathy is a major complication of diabetes mellitus.
  • C-peptide has shown renoprotective effects in diabetic models.
  • The mechanism may involve modulation of renal Na(+)/K(+)-ATPase activity.

Purpose of the Study:

  • To investigate the effect of C-peptide on Na(+)/K(+)-ATPase expression in the kidneys of diabetic rats.
  • To clarify the role of C-peptide in regulating renal Na(+)/K(+)-ATPase in diabetes.

Main Methods:

  • Type 1 diabetes was induced in rats using streptozotocin (STZ).
  • Diabetic rats were treated with C-peptide or insulin for one week.
  • Kidney expression of Na(+)/K(+)-ATPase alpha1, alpha2, and beta3 subunits was analyzed using mRNA and immunohistochemistry.

Main Results:

  • Diabetes significantly decreased Na(+)/K(+)-ATPase alpha1-subunit mRNA and protein expression in the renal medulla.
  • Insulin treatment did not prevent the decrease in alpha1-subunit expression.
  • C-peptide treatment prevented the diabetes-induced reduction in Na(+)/K(+)-ATPase alpha1-subunit expression.
  • Na(+)/K(+)-ATPase beta3-subunit expression was not significantly affected by diabetes or treatments.

Conclusions:

  • Diabetes selectively downregulates Na(+)/K(+)-ATPase alpha1-subunit expression in the kidney.
  • C-peptide administration preserves Na(+)/K(+)-ATPase alpha1-subunit expression in diabetic rats.
  • C-peptide may have a long-term protective role in regulating renal Na(+)/K(+)-ATPase function during diabetes.