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Updated: Jun 15, 2026
06:24
Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
Toward design-based engineering of industrial microbes
Keith E J Tyo1, Kanokarn Kocharin, Jens Nielsen
1Department of Chemical and Biological Engineering, Chalmers University of Technology, Göteborg SE-412 96, Sweden.
Current Opinion in Microbiology
|March 16, 2010
Summary
Industrial microbe engineering advances with systems biology and synthetic biology, moving towards a design-based approach rather than iterative methods for better cell biology understanding and goal achievement.
Area of Science:
- Microbial Engineering
- Systems Biology
- Synthetic Biology
Background:
- Engineering industrial microbes is limited by incomplete cell biology knowledge.
- An iterative cycle of modeling, implementation, and analysis has been the standard approach.
- Recent advances in Systems Biology and Synthetic Biology offer new possibilities.
Purpose of the Study:
- To describe the transition of microbial engineering from an iterative to a design-oriented paradigm.
- To highlight key technologies enabling this shift.
- To discuss the implications for future microbial engineering.
Main Methods:
- Review of recent advances in Systems Biology technologies.
- Integration of Synthetic Biology tools for modeling and molecular implementation.
- Discussion of genome-scale metabolic models, gene expression control tools, and integrated -omics analysis.
Main Results:
- Systems Biology technologies significantly increase data collection per iteration.
- Synthetic Biology tools merge modeling with molecular implementation.
- Key technologies identified to facilitate a design-based approach.
Conclusions:
- Advances in Systems Biology and Synthetic Biology are transforming microbial engineering.
- The field is shifting towards a design-based paradigm, akin to engineering disciplines like electrical circuits.
- This paradigm shift promises more efficient and predictable microbial engineering outcomes.
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