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Updated: Jun 15, 2026
Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody
Published on: May 16, 2020
[Expression, purification, antibody preparation and identification of tumor protein translationally-controlled 1
Yi-fan Zhao1, Hai-fang Li, Shi-ming Tang
1Tianjin Life Science Research Center, Basic Medical School, Tianjin Medical University, Tianjin, China.
Aim:
To express and purify the fusion protein of TPT1 (tumor protein translationally-controlled 1) in prokaryocytes and to prepare rabbit anti-TPT1 antibody.
Methods:
The expression vector pRSETA(2)-TPT1 was reconstructed and transformed into BL21 (DE3). TPT1 fusion protein was induced by IPTG and the TPT1 fusion protein purified by Ni-NTA His Bind resin was used to immunize the rabbit. The titer of the polyclonal antibody was detected by ELISA and its specificity was analyzed by Western blot and IF (immunofluorescence).
Results:
The TPT1 fusion protein was highly expressed in E.coli and specific polyclonal antibody was obtained after the immunization.
Conclusion:
The recombinant prokaryotic expression vector pRSETA(2)-TPT1 is successfully constructed and high expression of TPT1 is induced in E.coli, which may lay the basis for further study of the development and treatment of tumors and other diseases.
