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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent
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Peptide heterodimers for molecular imaging.

Yongjun Yan1, Xiaoyuan Chen

  • 1Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA.

Amino Acids
|March 17, 2010
PubMed
Summary

Peptide heterodimers, linking two different ligands, significantly enhance tumor targeting affinity and retention time for molecular imaging probes compared to single peptides. This approach shows promise for multi-receptor overexpressed tumors.

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Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Published on: October 20, 2018

Area of Science:

  • Molecular imaging
  • Bioconjugate chemistry
  • Oncology

Background:

  • Peptide-based molecular imaging probes often suffer from low tumor affinity and short retention.
  • Multivalency, using multiple ligands, can improve binding affinity.
  • Traditional multivalency uses identical peptides (homodimers/homomultimers).

Purpose of the Study:

  • To review peptide homodimers and heterodimers for molecular imaging.
  • To emphasize the potential of peptide heterodimers for enhanced tumor targeting.
  • To discuss the mechanisms behind improved binding affinity with heterodimers.

Main Methods:

  • Review of existing literature on peptide dimers in molecular imaging.
  • Analysis of binding modes (monovalent, bivalent) of peptide heterodimers.
  • Discussion of linker strategies and receptor targeting principles.

Main Results:

  • Peptide heterodimers, combining two distinct ligands, show significant affinity enhancement over monomers.
  • Both monovalent and bivalent binding modes of heterodimers improve binding kinetics and local ligand concentration.
  • Multivalency effect further contributes to binding in the bivalent mode.

Conclusions:

  • Peptide heterodimers represent a promising strategy to overcome limitations of current molecular imaging probes.
  • Their ability to target multiple overexpressed receptors on tumor cells offers improved diagnostic potential.
  • Further development of peptide heterodimer-based probes is anticipated for advanced molecular imaging applications.