Related Experiment Video
Updated: Jun 15, 2026
05:42
Three-Dimensional Cell Culture of Adipose-Derived Stem Cells in a Hydrogel with Photobiomodulation Augmentation
Published on: April 5, 2024
Laser biomodulation on L 929 cell culture
Deise A A Pires-Oliveira1, Rodrigo F Oliveira, Aline H A Machado
1Universidade Camilo Castelo Branca, Unicastelo, São Paulo, Brazil.
Photomedicine and Laser Surgery
|March 18, 2010
Summary
Photobiomodulation using a 904-nm diode laser enhanced mitochondrial activity and endoplasmic reticulum function in L929 fibroblast cells. Higher energy density (50 mJ/cm(2)) showed slightly better results, with effects being time-dependent.
Area of Science:
- Cell biology
- Biophotonics
- Laser therapy
Background:
- Low-power laser irradiation (LPLI) offers a non-pharmacological approach for in vitro photobiomodulation.
- LPLI influences cell cultures and tissues, prompting investigation into its cellular mechanisms.
Purpose of the Study:
- To investigate the effects of 904-nm diode laser photobiomodulation on L929 fibroblast cells.
- To analyze the impact of two distinct energy densities (6 J/cm(2) and 50 mJ/cm(2)) on cellular responses.
Main Methods:
- L929 fibroblast cells were irradiated with a 904-nm diode laser over three days at 24-hour intervals.
- Mitochondrial activity and endoplasmic reticulum changes were assessed using MitoTracker Orange and DioC6 staining.
- Cell proliferation was evaluated using the MTT assay.
Main Results:
- Irradiation with both 6 J/cm(2) and 50 mJ/cm(2) resulted in intense mitochondrial activity, confirmed by DioC6 staining.
- Increased protein synthesis led to observable reticular activity in the endoplasmic reticulum.
- Photobiomodulation at 50 mJ/cm(2) yielded slightly superior results compared to 6 J/cm(2).
Conclusions:
- Photobiomodulation effectively enhances mitochondrial and endoplasmic reticulum activity in fibroblasts.
- The observed cellular effects are dependent on laser energy density and time post-irradiation, with optimal results at 72 hours.
- This study highlights the potential of specific laser parameters for modulating cellular functions.
