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Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
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Serologic survey for bovine pathogens in free-ranging European bison from Poland

J Kita1, K Anusz

  • 1Department of Infectious Diseases, Faculty of Veterinary Medicine, Warsaw Agricultural University, Poland.

Journal of Wildlife Diseases
|January 1, 1991
PubMed

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Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.