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Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Related Experiment Video

Updated: Jun 15, 2026

DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis

DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis

Published on: September 28, 2021

A combination method for preparing casting and transparent liver specimen.

Nan Zheng¹, Jin Gong, Sheng-Bo Yu

¹Department of Anatomy, Dalian Medical University, Liaoning, China.

Clinical Anatomy (New York, N.Y.)

|March 18, 2010

Summary

A new method combining corrosion casting and transparency techniques for rabbit livers simultaneously visualizes intrahepatic vessels and liver structures. This approach enhances understanding of liver anatomy for surgical and radiological applications.

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Last Updated: Jun 15, 2026

DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis

DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis

Published on: September 28, 2021

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Published on: October 25, 2012

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Area of Science:

Hepatic Surgery
Radiology
Anatomical Pathology

Background:

Advancements in hepatic surgery and radiology necessitate a clearer understanding of intrahepatic vessels and liver segments.
Traditional research methods like dissection and corrosion casting have limitations in visualizing both internal and external liver structures concurrently.

Purpose of the Study:

To develop a novel specimen preparation technique for simultaneously demonstrating intrahepatic vessels and the liver's external structures.
To improve the visualization of the intricate relationships between internal vascular anatomy and external liver morphology.

Main Methods:

Rabbit livers were subjected to three preparation techniques: corrosion-casting, transparency, and a combination of both.
Evaluation focused on the clarity of visualization for both intrahepatic vessels and external liver anatomy.

Main Results:

The combined corrosion-casting and transparency technique (Group C) provided clear visualization of both intrahepatic vessels and external liver structures.
This combined method effectively revealed the spatial relationships between the internal vascular network and the liver's external features.

Conclusions:

The developed combination method offers a new approach for studying intrahepatic vessels.
This technique is particularly valuable for applied basic research in hepatic segmentation and surgical planning.