Related Experiment Video
Updated: Jun 15, 2026
Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model
Published on: March 17, 2023
Evaluation of thyroglobulin expression in murine reproductive organs during pregnancy
Ali Moravej1, Mahmood Jeddi-Tehrani, Ali R Salek-Moghaddam
1Immunology Research Center, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Problem:
In pregnant women with antithyroglobulin antibody, prevalence of abortion is 2-4 fold higher compared to normal controls. Direct effect of such harmful autoantibodies on female reproductive organs may serve a role in pregnancy loss.
Method Of Study:
Expression of thyroglobulin in decidua, placenta, and ovary of pregnant Balb/c mice ((Balb/cxBalb/c and Balb/cxC57BL/6) during early, middle, and late stages of pregnancy was evaluated. Expression of thyroglobulin was investigated in these tissues by semi-quantitative RT-PCR. In addition, polyclonal antithyroglobulin antibody was produced, and expression of thyroglobulin protein in aforesaid tissues was evaluated by immunohistochemistry and dot-blot analysis.
Results:
The results showed that thyroglobulin message is not expressed in placenta, decidua, or ovary in any stages of pregnancy. The same results were obtained at the protein level.
Conclusion:
It is likely that antithyroglobulin antibodies have no direct detrimental effect on such organs in patients with thyroid autoimmunity suffering from recurrent abortion.
