Related Experiment Video

Updated: Jan 8, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

Published on: January 20, 2015

9.2K

Preferential association of newly synthesized histones with replicating SV40 DNA

C Crémisi, A Chestier, M Yaniv

    Cell
    |December 1, 1977
    PubMed

    More Related Videos

    Primary Culture of Mouse Dopaminergic Neurons11:58

    Primary Culture of Mouse Dopaminergic Neurons

    Published on: September 8, 2014

    39.1K
    Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area09:54

    Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

    Published on: August 10, 2012

    26.4K

    Related Concept Videos

    Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons09:21

    Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons

    This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical...
    2.0K
    Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression10:54

    Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

    In Parkinson's Disease (PD), Substantia Nigra (SNc) dopaminergic neurons degenerate, leading to motor dysfunction. Here we report a protocol for culturing ventral midbrain neurons from a mouse expressing eGFP driven by a Tyrosine Hydroxylase (TH) promoter sequence, harvesting individual fluorescent neurons from the cultures, and measuring their transcriptome using...
    10.0K
    Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains08:45

    Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

    The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of...
    14.5K
    Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice09:35

    Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

    This protocol describes two different environmental manipulations and a concurrent brain infusion protocol to study environmentally-induced brain changes underlying adaptive behavior and brain repair in adult...
    9.2K
    Primary Culture of Mouse Dopaminergic Neurons11:58

    Primary Culture of Mouse Dopaminergic Neurons

    Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various...
    39.1K
    Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area09:54

    Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

    Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in...
    26.4K