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Updated: Aug 13, 2026

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. I. Collection of Virus Samples
Published on: March 28, 2015
Persistence of enteroviruses in sewage sludge
Most viruses in sewage sludge can survive for weeks, posing a risk for land application. Adequate virus inactivation treatment is crucial for sewage sludge recycling programs to ensure public health and safety.
Area of Science:
- Environmental microbiology
- Virology
- Public health
Background:
- Residential sewage contains human pathogenic viruses.
- Viruses in sewage sludge pose risks during land application.
- Data on virus survival in sludge is needed for safe recycling guidelines.
Purpose of the Study:
- To determine the survival rate of enteroviruses in sewage sludge.
- To inform guidelines for land application of treated sewage sludge.
Main Methods:
- Artificial contamination of sewage sludge with enteroviruses.
- Monitoring virus survival over a 12-week period at room temperature.
Main Results:
- Most tested enteroviruses survived for many weeks in sewage sludge.
- Virus inactivation requires adequate treatment before land application.
Conclusions:
- Sewage sludge requires effective virus inactivation before land application.
- Hepatitis A virus and poliovirus survival may be similar.
- Public health risks necessitate stringent sludge treatment protocols.
More Related Videos
11:09EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
Published on: September 11, 2016
12:32EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
Published on: January 16, 2016
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