12-Hydroxyeicosatetraenoic acid (12-HETE) induces heat shock proteins in human leukocytes

12-Hydroxyeicosatetraenoic acid (12-HETE) induces the expression of individual heat shock proteins in human leukocytes (lymphocytes, monocytes, basophil granulocytes; LMBs). Metabolic radiolabeling of LMBs revealed that exogenous 12-HETE (20 ng) led to the expression of a 65- and 83kDa protein. Immunoreactivity towards the 65kDa protein was commonly detected. In contrast, after heat shock treatment and predominantly after incubation with 12-HETE significant immunoreactivity (anti-hsp72) was detected in the lower molecular weight range whereas immunoreactivity (anti-hsp90, AC88) was only observed after heat shock treatment of LMBs.