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Updated: Jun 14, 2026
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
Published on: March 1, 2013
Folate-PEG modified poly(2-(2-aminoethoxy)ethoxy)phosphazene/DNA nanoparticles for gene delivery: synthesis,
Pengcheng Zhang1, Zhiwen Zhang, Yongxin Yang
1Center for Drug Delivery System, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
Abstract:
Target-specific technique can significantly enhance the efficacy of gene delivery system which was limited by many cellular barriers. In this work, a new folate-PEG modified poly(2-(2-aminoethoxy)ethoxy)phosphazene (PAEP), namely, folate-PEG-PAEP was synthesized as a folate receptor (FR) targeted carrier, and the cytotoxicity, transfection efficiency, cellular uptake and intracellular trafficking of folate-PEG-PAEP/DNA nanoparticles (FPPN) were investigated. Compared with the PAEP/DNA nanoparticles (PN), the cytotoxicity of FPPN decreased significantly at high dose. FPPN showed much higher transfection efficiency (15.85+/-1.23%) compared with PN (6.71+/-0.42%) in FR overexpressing Hela cells, but no significant difference was observed in CHO-k1 cells lacking FR. The transfection activity of FPPN could be reversed in the presence of 1.0mM free folic acid in Hela cells. The cellular uptake of FPPN was 37.38% higher than that of PN in Hela cells. These results indicated that FPPN could be a potential targeted gene delivery system.
