Coupling liquid chromatography to Orbitrap mass spectrometry.
Alexander Makarov1, Michaela Scigelova
1Thermo Fisher Scientific, Hanna-Kunath-Str. 11, 28199 Bremen, Germany. Alexander.makarov@thermofisher.com
Journal of Chromatography. A
|March 20, 2010
Summary
The Orbitrap mass analyzer is a key technique in mass spectrometry. This review covers its applications with various chromatography methods, especially liquid chromatography (LC), for analyzing small molecules and proteins.
Area of Science:
- Mass Spectrometry
- Analytical Chemistry
- Chromatography
Background:
- The Orbitrap mass analyzer is a widely adopted technique in modern mass spectrometry.
- Its high resolution and mass accuracy have made it a preferred choice for various analytical challenges.
Purpose of the Study:
- To review recent publications on Orbitrap mass analyzer applications.
- To highlight its integration with diverse chromatographic separation techniques.
Main Methods:
- Literature review of recent publications.
- Focus on Orbitrap detection coupled with various chromatography methods.
Main Results:
- Orbitrap coupled with reversed-phase liquid chromatography (LC) is common for small molecule and proteomic analyses.
- Multi-dimensional LC is crucial for proteomics.
- Ultra-high-pressure LC is prevalent in metabolomics.
- Hydrophilic interaction chromatography (HILIC) and its variations are increasingly used with Orbitrap.
Conclusions:
- The Orbitrap mass analyzer demonstrates versatility across numerous chromatographic techniques.
- Its combination with LC, particularly reversed-phase and multi-dimensional LC, is essential for complex biological sample analysis.
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