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Updated: Jun 14, 2026

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

High-throughput fluorescence detection using an integrated zone-plate array.

Ethan Schonbrun¹, Adam R Abate, Paul E Steinvurzel

¹School of Engineering and Applied Sciences, Harvard University, Cambridge, USA. schonbru@seas.harvard.edu

|March 20, 2010

Summary

This summary is machine-generated.

This study introduces a novel fluorescence measurement platform that integrates microfluidics with optics. This innovation enables high-throughput analysis of nearly 200,000 drops per second, overcoming limitations of existing detection systems.

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Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

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Published on: January 18, 2017

Area of Science:

Optics and Photonics
Microfluidics
Biotechnology

Background:

Microfluidic devices offer massive parallelization for sample manipulation.
Existing optical detection systems lack the parallelization and integration needed for microfluidic arrays.
Current optical systems struggle with the field of view and sensitivity for microfluidic applications.

Purpose of the Study:

To develop a parallelized and integrated optical detection system for microfluidic devices.
To overcome the limitations of standard optical systems in microfluidic high-throughput analysis.
To present a novel fluorescence measurement platform for microfluidic applications.

Main Methods:

Development of a microfluidic device with 64 output channels.
Integration of a microfabricated zone-plate array for optical detection.
Orientation of the zone-plate array for parallel readout of fluorescence signals.

Main Results:

A novel fluorescence measurement platform integrating microfluidics and optics was created.
The system utilizes a zone-plate array for parallel detection.
The integrated system achieves analysis of nearly 200,000 drops per second.

Conclusions:

The presented platform successfully integrates microfluidics and optics for high-throughput analysis.
This approach overcomes the limitations of conventional optical detection systems for microfluidic applications.
The developed system enables unprecedented analysis rates in microfluidic devices.