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Updated: Jun 14, 2026

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
Published on: July 20, 2019
Imaging of organelles by electron microscopy reveals protein-protein interactions in mitochondria and chloroplasts
Natalya V Dudkina1, Roman Kouril, Jelle B Bultema
1Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.
Abstract:
Ongoing progress in electron microscopy (EM) offers now an opening to visualize cells at the nanoscale by cryo-electron tomography (ET). Large protein complexes can be resolved at near-atomic resolution by single particle averaging. Some examples from mitochondria and chloroplasts illustrate the possibilities with an emphasis on the membrane organization. Cryo-ET performed on non-chemically fixed, unstained, ice-embedded material can visualize specific large membrane protein complexes. In combination with averaging methods, 3D structures were calculated of mitochondrial ATP synthase at 6 nm resolution and of chloroplast photosystem II at 3.5 nm.
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