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Updated: Jun 14, 2026

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
Babesia bovis expresses a neutralization-sensitive antigen that contains a microneme adhesive repeat (MAR) domain
Marta G Silva1, Massaro W Ueti, Junzo Norimine
1Biomolecular Diagnostic Laboratory, ITQB/IBET, Universidade Nova de Lisboa, Av. da Republica, Apt 12, Oeiras, Portugal. msilva@itqb.unl.pt
Abstract:
A gene coding for a protein with sequence similarity to the Toxoplasma gondii micronemal 1 (MIC1) protein that contains a copy of a domain described as a sialic acid-binding micronemal adhesive repeat (MAR) was identified in the Babesia bovis genome. The single copy gene, located in chromosome 3, contains an open reading frame encoding a putative 181 amino acid protein, which is highly conserved among distinct B. bovis strains. Antibodies against both recombinant protein and synthetic peptides mimicking putative antigenic regions in the B. bovis-MIC1 (Bbo-MIC1) protein bind to the parasite in immunofluorescence assays and significantly inhibit erythrocyte invasion in in vitro B. bovis cultures. Bbo-MIC1 is recognized by antibodies in serum from B. bovis infected cattle, demonstrating expression and immunogenicity during infection. Overall, the results suggest that Bbo-MIC1 protein is a viable candidate for development of subunit vaccines.
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