Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same authorSame Topic

An encyclopedia of human enhancer-gene regulatory interactions.

Nature·2026
Same author

Advancing Functional Transcriptomics in Zebrafish with High-accuracy Full-length RNA Sequencing.

Genomics, proteomics & bioinformatics·2026
Same author

The Earth BioGenome Project Phase II: illuminating the eukaryotic tree of life.

Frontiers in science·2026
Same author

Overcoming the widespread flaws in the annotation of vertebrate selenoprotein genes in public databases.

PLoS computational biology·2026
Same author

An expanded registry of candidate cis-regulatory elements.

Nature·2026
Same author

Long-read transcriptomics of a diverse human cohort reveals ancestry bias in gene annotation.

Nature communications·2025

Related Experiment Video

Updated: Jun 14, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

From chromatin to splicing: RNA-processing as a total artwork.

Hagen Tilgner1, Roderic Guigó1

  • 1Universitat Pompeu Fabra.

Epigenetics
|March 23, 2010
PubMed
Summary

Chromatin structure, including nucleosome positioning and histone modifications, influences RNA processing and pre-messenger RNA splicing. This suggests chromatin state directly regulates gene expression and cellular phenotype.

Area of Science:

  • Molecular Biology
  • Genomics
  • Epigenetics

Background:

  • RNA is crucial for cellular phenotype determination.
  • Mechanisms of RNA synthesis and processing are not fully understood and appear increasingly coupled.
  • Recent genome-wide chromatin maps suggest a role for chromatin structure in RNA processing.

Purpose of the Study:

  • To investigate the role of chromatin structure in RNA processing.
  • To explore the link between nucleosome positioning and exon recognition in pre-mRNA splicing.
  • To examine the potential regulatory role of chromatin state in splicing.

Main Methods:

  • Analysis of genome-wide chromatin structure maps.
  • Examination of nucleosome occupancy patterns in metazoan exonic regions.

More Related Videos

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Related Experiment Videos

Last Updated: Jun 14, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

  • Observation of histone modification patterns in exonic regions.

    • Main Results:

    • Nucleosomes exhibit characteristic occupancy patterns in exonic regions of metazoan genomes.
    • These patterns suggest nucleosome positioning is involved in exon recognition during pre-mRNA splicing.
    • Specific exonic patterns of histone modifications were observed, indicating a role for chromatin state.

    Conclusions:

    • Primary chromatin structure plays a significant role in RNA processing.
    • Nucleosome positioning is implicated in pre-mRNA splicing and exon recognition.
    • Chromatin state may directly regulate RNA splicing and, consequently, cellular phenotype.