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Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

¹³C NMR: ¹H–¹³C Decoupling

¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...

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Related Experiment Video

Updated: Jun 14, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Singular Value Decomposition-based Alternative Splicing Detection.

Jianhua Hu¹, Xuming He, Gilbert J Cote

¹Department of Biostatistics, University of Texas M. D. Anderson Cancer Center, Houston, TX.

Journal of the American Statistical Association

|March 23, 2010

Summary

This summary is machine-generated.

A new singular value decomposition (SVD) method enhances the detection of alternative splicing events in brain cancer using exon tiling array data. This approach improves identification of cancer-related splicing events and associated genomic regions.

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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Area of Science:

Genomics
Bioinformatics
Cancer Research

Background:

Altered alternative splicing is a key factor in tumorigenesis.
Existing analysis tools for exon tiling arrays have limitations in detecting alternative splicing events.
Transcriptome-wide analysis of alternative splicing is crucial for understanding cancer development.

Purpose of the Study:

To develop and evaluate a novel screening procedure for detecting alternative splicing events.
To improve the analysis of exon tiling array data for brain cancer studies.
To identify probe selection regions (PSRs) associated with alternative splicing.

Main Methods:

Proposed a singular value decomposition (SVD)-based screening procedure.
Utilized a robust additive model fit to probe selection region (PSR) data.
Analyzed exon tiling array data from a brain cancer study.

Main Results:

The SVD-based approach effectively accommodates outlying measures in gene expression data.
The method capitalizes on multidimensional gene expression profiles for improved detection.
Identified group-specific alternative splicing events and associated PSRs with higher accuracy.

Conclusions:

The proposed SVD-based method offers a more effective approach for detecting alternative splicing events.
Findings provide a valuable list of candidate alternative splicing events for further investigation.
The method enhances the utility of exon tiling arrays in cancer research.