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Vinblastine-induced numerical chromosome changes and selection processes in mouse bone marrow cells.
B Gustavino1, B Bassani, F Pacchierotti
1Laboratory of Toxicology, ENEA, CRE Casaccia, Rome, Italy.
Mutation Research
|May 1, 1991
Summary
Vinblastine treatment in mice induced aneuploid and polyploid cells. These cells, particularly polyploid ones, showed longer cell cycles and reduced mitosis probability in later generations.
Area of Science:
- Cell biology
- Genetics
- Toxicology
Background:
- Chemically induced aneuploidy and polyploidy can arise from agents like vinblastine (VBL).
- Understanding the long-term effects of these chromosomal abnormalities on cell populations is crucial.
Purpose of the Study:
- To investigate the impact of selection processes on chemically induced aneuploid and polyploid mouse bone marrow cells.
- To analyze cell-cycle progression and mitotic probability of these abnormal cells over generations.
Main Methods:
- Mouse bone marrow cells were treated with vinblastine (VBL) and analyzed over three generations.
- Bromodeoxyuridine (BrdUrd) labeling was used to track cell-cycle progression.
- Chromosome number analysis identified aneuploid and polyploid cells at different time points within the third cell cycle.
Main Results:
- Polyploid cells and cells with numerous extra chromosomes exhibited longer cell cycles compared to diploid cells.
- Both hyperploid and polyploid cells showed a decreased probability of undergoing further mitoses.
- Frequencies of hyperploid and polyploid cells were reduced in the third cell cycle post-VBL treatment.
Conclusions:
- Selection processes favor diploid cells, leading to a decrease in aneuploid and polyploid cell populations over generations.
- Altered cell-cycle kinetics and reduced mitotic success contribute to the elimination of chromosomally abnormal cells.