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Production of Organic Acids01:25

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...

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Related Experiment Video

Updated: Jun 14, 2026

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
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Purification of lactoferrin using hydroxyapatite.

Paul K Ng1, Tomohiko Yoshitake

  • 1Process Applications R&D, Process Chromatography Division, Bio-Rad Laboratories, Hercules, CA 94547, USA. paul ng@bio-rad.com

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|March 24, 2010
PubMed
Summary
This summary is machine-generated.

This study presents a novel chromatography method to isolate lactoferrin (LF) from whey. The technique efficiently separates LF from other whey proteins, yielding a pure nutriceutical product.

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Area of Science:

  • Biochemistry
  • Chromatography
  • Food Science

Background:

  • Lactoferrin (LF) is a valuable nutriceutical with diverse physiological roles.
  • LF is found in low concentrations within whey, necessitating efficient isolation methods.
  • Separating LF from similar proteins like lactoperoxidase presents a challenge.

Purpose of the Study:

  • To develop a single-column chromatography method for efficient lactoferrin fractionation from whey.
  • To achieve high purity lactoferrin, free from major whey protein contaminants.

Main Methods:

  • Utilized mixed-mode (hydroxyapatite) chromatography for whey fractionation.
  • Employed isocratic elution conditions to selectively desorb proteins.
  • Optimized conditions to separate lactoferrin from lactoperoxidase and other whey proteins.

Main Results:

  • Successfully isolated lactoferrin in a single chromatographic step.
  • Achieved lactoferrin purity, free from lactoperoxidase activity.
  • Demonstrated separation from other major whey proteins, including alpha lactalbumin and beta lactoglobulin.

Conclusions:

  • Mixed-mode hydroxyapatite chromatography is an effective method for lactoferrin purification from whey.
  • This technique offers a streamlined approach to obtaining high-purity lactoferrin.
  • The method provides a valuable tool for the nutriceutical and food industries.