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Related Concept Videos

Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...
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Updated: Jun 14, 2026

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
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Heparanase in primary human osteoblasts.

Paul N Smith1, Craig Freeman, Di Yu

  • 1Medical School, The Australian National University, Canberra, Australia.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
|March 24, 2010
PubMed
Summary
This summary is machine-generated.

Heparanase (HPSE) is expressed in human osteoblasts and plays a role in bone formation. Lower HPSE levels in osteoporosis suggest it may be a therapeutic target for bone disease.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Orthopedics

Background:

  • Heparanase (HPSE) is implicated in fracture repair in mice.
  • Its role in human bone formation and osteogenesis is not well understood.

Purpose of the Study:

  • To investigate HPSE expression and function in human osteoblasts.
  • To elucidate the role of HPSE in osteogenesis and its potential link to osteoporosis.

Main Methods:

  • Isolated osteoblasts from human trabecular bone of osteoporosis patients and healthy subjects.
  • Assessed HPSE protein expression, enzymatic activity, and gene expression.
  • Analyzed alkaline phosphatase (ALP) activity and osteogenic gene profiles.
  • Investigated the effect of exogenous HPSE on histone H3 phosphorylation.

Main Results:

  • HPSE protein and enzymatic activity were detected in human osteoblasts, with lower levels in osteoporotic osteoblasts.
  • HPSE gene expression correlated with ALP activity, a bone turnover marker.
  • Osteoporotic osteoblasts showed downregulated osteogenic genes.
  • Exogenous HPSE increased histone H3 phosphorylation in osteoblasts.

Conclusions:

  • HPSE is expressed and functional in human osteoblasts, influencing osteoblastogenesis.
  • HPSE regulates osteogenic gene expression and histone H3 modification.
  • HPSE upregulation presents a potential therapeutic strategy for osteoporosis prevention and treatment.