Haptoglobin typing from fatty tissues after extraction with non-ionic detergents

Of the seven non-ionic detergents tested, Span 40, Tween 20 and Triton X-45 were suitable for extraction of haptoglobin (Hp) from fatty tissues. The extracted samples were subjected to electrophoresis and subsequently stained with o-tolidine. Enzyme-immunoassay and Western blotting (electrophoretic) techniques were used successfully to determine the Hp phenotypes from minute quantities of tissue samples after extractions with non-ionic detergents.