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Updated: Jun 14, 2026
Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts
Published on: May 13, 2014
Spontaneous in vitro transformation of primary human osteoblast-like cells
Ursula Magdolen1, Manfred Schmitt, Barbara Hildebrandt
1Department of Orthopedic Surgery, Klinikum rechts der Isar, Technische Universitaet Muenchen, D-81675 Muenchen, Germany.
Background:
Two new tumor-like cell lines were established which developed spontaneously in vitro from normal human primary osteoblast-like cells originating from non-oncogenic bone surgery.
Materials And Methods:
The tumor cell properties studied included morphology, proliferation characteristics in normal and low-serum media, and anchorage-independent growth in soft agarose.
Results And Conclusion:
Karyotyping of the cells showed numerous rearrangements and abnormalities. These results pointed to the tumorigenic potential of the cells and demonstrate the importance of biosafety in tissue engineering and therapeutic cell applications when prolonged culture conditions are required.
