RP-HPLC Estimation of Ramipril and Telmisartan in Tablets.

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis

Background:

• Ramipril and telmisartan are commonly prescribed antihypertensive agents.
• Accurate quantification of active pharmaceutical ingredients in combined dosage forms is crucial for therapeutic efficacy and patient safety.

Purpose of the Study:

• To develop and validate a rapid, simultaneous, and precise analytical method for ramipril and telmisartan in their combined dosage form.
• To establish a robust chromatographic method suitable for routine quality control of pharmaceutical formulations.

Main Methods:

• High-performance liquid chromatography (HPLC) using a Genesis C18 column.
• Isocratic elution with a mobile phase of potassium dihydrogen phosphate buffer, methanol, and acetonitrile.
• Detection at 210 nm with a flow rate of 1.0 mL/min.

Main Results:

• The method achieved effective separation of ramipril (Rt: 3.68 min) and telmisartan (Rt: 4.98 min) with a resolution of 3.84.
• Excellent linearity was observed within the ranges of 3.5-6.5 µg/mL for ramipril and 28.0-52.0 µg/mL for telmisartan.
• High percentage recoveries (99.09-101.64% for ramipril, 99.45-100.99% for telmisartan) and low limits of detection/quantitation demonstrate method accuracy and sensitivity.

Conclusions:

• The developed HPLC method is rapid, accurate, precise, and robust for simultaneous estimation of ramipril and telmisartan.
• This validated analytical technique is suitable for quality control and assay of ramipril and telmisartan in combined pharmaceutical formulations.