Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A genome-scale CRISPRi perturbation atlas of human induced pluripotent stem cells.

Nature biotechnology·2026
Same author

Receptor-Guided AAV Tropism Engineering via MATCH.

ACS synthetic biology·2026
Same author

Efficient and robust generation of functional hematopoietic cells from human pluripotent stem cells in albumin-free conditions.

Stem cells translational medicine·2026
Same author

Receptor-guided AAV Tropism Engineering via MATCH.

bioRxiv : the preprint server for biology·2026
Same author

Human Dorsal Root Ganglia Neuronal Cell Line to Study Nociceptive Signaling: A New Pipeline for Pain Therapy.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Multiplexed Pan Soluble Ligandome Assaying via OASIS.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jun 14, 2026

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells
13:18

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells

Published on: June 28, 2013

An improved method for generating and identifying human induced pluripotent stem cells.

Prashant Mali1, Zhaohui Ye, Bin-Kuan Chou

  • 1Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 26, 2010
PubMed
Summary
This summary is machine-generated.

This study details methods for deriving and characterizing human induced pluripotent stem (iPS) cells. These techniques enable the generation and identification of iPS cells from fibroblast populations using reprogramming genes.

More Related Videos

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
09:43

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Published on: April 23, 2014

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector
12:03

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

Published on: October 31, 2012

Related Experiment Videos

Last Updated: Jun 14, 2026

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells
13:18

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells

Published on: June 28, 2013

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
09:43

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Published on: April 23, 2014

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector
12:03

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

Published on: October 31, 2012

Area of Science:

  • Stem Cell Biology
  • Molecular Biology
  • Cellular Reprogramming

Background:

  • Human induced pluripotent stem (iPS) cells hold significant potential for regenerative medicine and disease modeling.
  • Efficient and reliable methods for iPS cell derivation are crucial for their widespread application.
  • Current protocols require detailed understanding of cell culture, viral vector preparation, and reprogramming gene expression.

Purpose of the Study:

  • To provide a comprehensive protocol for deriving and characterizing human iPS cells.
  • To outline methods for fibroblast culture, retrovirus preparation, and iPS cell induction.
  • To describe techniques for monitoring, identifying, and characterizing resulting iPS cell lines.

Main Methods:

  • Fibroblast cell culture and maintenance.
  • Retrovirus preparation, concentration, and transduction using vectors encoding reprogramming factors (OCT4, SOX2, KLF4, c-MYC or fewer).
  • Live cell staining for monitoring and identification of reprogrammed colonies.
  • Characterization of established iPS cell lines.

Main Results:

  • A detailed protocol for human iPS cell derivation from fibroblasts is presented.
  • Methods for efficient retroviral gene delivery and reprogramming are described.
  • A robust live-staining technique facilitates the identification of potential iPS cell colonies.
  • Established protocols for comprehensive characterization of derived iPS cell lines are outlined.

Conclusions:

  • The described methods provide a reliable framework for generating and validating human iPS cells.
  • This protocol supports advancements in stem cell research and therapeutic applications.
  • Standardized techniques ensure consistent and reproducible iPS cell derivation and characterization.