Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 14, 2026

Large Scale Zebrafish-Based In vivo Small Molecule Screen
07:03

Large Scale Zebrafish-Based In vivo Small Molecule Screen

Published on: December 30, 2010

Zebrafish small molecule screen in reprogramming/cell fate modulation.

Jing-Ruey J Yeh1, Kathleen M Munson

  • 1Developmental Biology Laboratory, Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, MA, USA. jyeh1@partners.org

Methods in Molecular Biology (Clifton, N.J.)
|March 26, 2010
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MIC-Drop: A platform for large-scale in vivo CRISPR screens.

Science (New York, N.Y.)·2021
Same author

Genetic deletion of gpr27 alters acylcarnitine metabolism, insulin sensitivity, and glucose homeostasis in zebrafish.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2020
Same author

Subsurface seawater methylmercury maximum explains biotic mercury concentrations in the Canadian Arctic.

Scientific reports·2018
Same author

Small Molecule Inhibitors of NFkB Reverse Iron Overload and Hepcidin Deregulation in a Zebrafish Model for Hereditary Hemochromatosis Type 3.

ACS chemical biology·2018
Same author

Dopaminergic control of anxiety in young and aged zebrafish.

Pharmacology, biochemistry, and behavior·2017
Same author

Extreme Vulnerability of IDH1 Mutant Cancers to NAD+ Depletion.

Cancer cell·2015
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

This study used zebrafish to show that the AML1-ETO oncogene can switch blood cell development. Researchers screened for drugs that could block this oncogene-induced cell fate change.

Area of Science:

  • Developmental Biology
  • Hematopoiesis
  • Cancer Biology

Background:

  • Embryonic zebrafish are valuable models for lineage-tracing studies.
  • Cell fate determination in zebrafish embryos can be achieved through in situ hybridization or fluorescent reporter lines.
  • Hematopoietic cell fate modulation is crucial in understanding blood disorders.

Purpose of the Study:

  • To investigate the role of the leukemic oncogene AML1-ETO in modulating hematopoietic cell fate in zebrafish.
  • To identify small molecules capable of suppressing AML1-ETO-induced cell fate switching.

Main Methods:

  • Utilized embryonic zebrafish as a model system.
  • Employed induced expression of AML1-ETO to reprogram hematopoietic progenitor cells.
  • Conducted a chemical screen to identify suppressor molecules.

More Related Videos

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos
07:45

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos

Published on: November 8, 2014

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae
02:52

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae

Published on: March 7, 2025

Related Experiment Videos

Last Updated: Jun 14, 2026

Large Scale Zebrafish-Based In vivo Small Molecule Screen
07:03

Large Scale Zebrafish-Based In vivo Small Molecule Screen

Published on: December 30, 2010

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos
07:45

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos

Published on: November 8, 2014

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae
02:52

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae

Published on: March 7, 2025

Main Results:

  • Demonstrated that AML1-ETO can reprogram erythroid progenitor cells to a myeloid cell fate.
  • Successfully identified small molecules that counteract the AML1-ETO-mediated cell fate switch.

Conclusions:

  • The zebrafish AML1-ETO model effectively demonstrates oncogene-induced cell fate reprogramming.
  • The chemical screen identified potential therapeutic compounds for modulating cell fate decisions.
  • The developed methods offer a broad applicability for discovering modulators of various cell fate pathways.