Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Triple-mode speciation of Fe<sup>2+</sup> and Fe<sup>3+</sup> using optical and paper-based digital imaging.

RSC advances·2026
Same author

Independent PML::RARA-positive acute promyelocytic leukemia arising during BCR::ABL1-positive chronic myeloid leukemia.

Journal of clinical and experimental hematopathology : JCEH·2026
Same author

Micropillar Topography Regulates Morphology and Melanogenesis in Melanoma Cells.

Journal of functional biomaterials·2026
Same author

The Effect of Closed Incision Negative Pressure Wound Therapy for Prophylactic Use of Postoperative Deep Sternal Wound Infection in Patients Following Coronary Artery Bypass Grafting: A Single-Institutional Study.

Annals of thoracic and cardiovascular surgery : official journal of the Association of Thoracic and Cardiovascular Surgeons of Asia·2026
Same author

Prophylactic closed-incision negative pressure wound therapy after median sternotomy: a scoping review of surgical site infection prevention bundle reporting and implication for interpretation.

The Journal of hospital infection·2026
Same author

Women in Japanese cardiovascular surgery: 21-year trends (2004-2025), current status and future perspectives.

General thoracic and cardiovascular surgery·2026

Related Experiment Video

Updated: Jun 14, 2026

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
09:04

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Published on: February 23, 2018

Developments and applications of mass microscopy.

Mitsutoshi Setou1, Kamlesh Shrivas, Morakot Sroyraya

  • 1Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan. setou@hama-med.ac.jp

Medical Molecular Morphology
|March 27, 2010
PubMed
Summary

Mass microscopy, combining microscopy with matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS), reveals biomolecule distribution in tissues. This technique aids in understanding cellular profiles and assessing medical molecular morphology for clinical applications.

More Related Videos

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
15:00

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Published on: June 14, 2016

Related Experiment Videos

Last Updated: Jun 14, 2026

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
09:04

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Published on: February 23, 2018

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
15:00

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Published on: June 14, 2016

Area of Science:

  • Biomedical Imaging
  • Analytical Chemistry
  • Molecular Pathology

Background:

  • Investigating biomolecule spatial distribution in biological tissues is crucial for understanding cellular functions and disease mechanisms.
  • Traditional methods for biomolecule analysis often involve complex and time-consuming extraction, purification, and separation steps.
  • There is a need for advanced techniques that can provide comprehensive molecular information directly from tissue sections.

Purpose of the Study:

  • To introduce and review the advancements in mass microscopy, a technique integrating microscopy with high-resolution matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS).
  • To highlight the capability of mass microscopy in visualizing the spatial distribution of hundreds of biomolecules within a single measurement.
  • To discuss the clinical applications of mass microscopy in assessing medical molecular morphology.

Main Methods:

  • Development of a mass microscopy technique combining optical microscopy with high-resolution MALDI-IMS.
  • Optimization of sample preparation protocols for enhanced biomolecule identification.
  • Focus on spatial resolution and measurement speed to improve analytical performance.

Main Results:

  • Mass microscopy enables direct visualization of biomolecule distribution in biological tissue sections without prior extraction or purification.
  • The technique provides clear imaging of hundreds of biomolecules in a single analytical run.
  • Demonstrated potential for assessing medical molecular morphology in clinical settings.

Conclusions:

  • Mass microscopy is a powerful tool for investigating the spatial distribution and cellular profiles of biomolecules.
  • Advancements in sample preparation, spatial resolution, and speed are critical for maximizing the technique's utility.
  • The reviewed clinical applications underscore the value of mass microscopy in medical diagnostics and research.