Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 14, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

Stable-isotope dilution LC–MS for quantitative biomarker analysis.

Eugene Ciccimaro1, Ian A Blair

  • 1Thermo Fisher Scientific, 265 Davidson Avenue, Somerset, NJ 08873–4120, USA.

Bioanalysis
|March 31, 2010
PubMed
Summary

Validated biomarker analysis is crucial for disease identification. Stable-isotope dilution (SID) LC–MS/MS offers superior analytical specificity for quantifying exposure and disease biomarkers, with future applications explored.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epigenetic reactivation in Friedreich's ataxia from benzamides to gene‑targeted chimeras.

Expert opinion on drug discovery·2026
Same author

Therapeutic activity of a hematopoietic stem cell-delivered cell-penetrating frataxin in Friedreich's ataxia models.

Cell reports. Medicine·2026
Same author

Safety and efficacy of individualised exercise and NAD<sup>+</sup> precursor supplementation in patients with Friedreich's ataxia in the USA: a single-centre, 2 × 2 factorial, randomised controlled trial.

The Lancet. Neurology·2026
Same author

Peripheral frataxin levels govern long-term clinical progression in Friedreich ataxia.

BMJ neurology open·2026
Same author

Author Correction: HMGB2-induced calreticulin translocation required for immunogenic cell death and ferroptosis of cancer cells are controlled by the nuclear exporter XPO1.

Communications biology·2025
Same author

Stable Isotope Labeling in Bacteria Enables Characterization and Quantification of Frataxin Protein in a Friedreich's Ataxia Zebrafish Model.

Analytical chemistry·2025

Area of Science:

  • Biomarker analysis
  • Analytical chemistry
  • Mass spectrometry

Background:

  • Validated biomarker analysis is essential for disease identification.
  • Accurate quantification of biomarkers requires high analytical specificity.
  • Stable-isotope dilution (SID) is a key technique in modern analytical chemistry.

Purpose of the Study:

  • To review the application of SID LC–MS methodology for biomarker analysis.
  • To highlight the importance of analytical specificity in biomarker validation.
  • To discuss future directions for SID LC–MS in biomarker research.

Main Methods:

  • Stable-isotope dilution (SID) methodology.
  • Liquid chromatography–tandem mass spectrometry (LC–MS/MS).
  • Analysis of small-molecule and protein biomarkers.

More Related Videos

Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS
07:12

Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS

Published on: August 23, 2024

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

Related Experiment Videos

Last Updated: Jun 14, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS
07:12

Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS

Published on: August 23, 2024

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

Main Results:

  • SID LC–MS/MS provides the highest analytical specificity for quantitative determinations.
  • This methodology is widely used for biomarker discovery and validation.
  • The review details the application of SID LC–MS for various biomarker types.

Conclusions:

  • SID LC–MS/MS is a powerful tool for rigorous biomarker analysis.
  • The methodology ensures high sensitivity and selectivity in disease biomarker identification.
  • Future directions include expanding the use of SID LC–MS for complex biomarker studies.