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Updated: Oct 24, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
Published on: May 27, 2014
Analytical Methods for Mass Spectrometry-Based Metabolomics Studies
Siyu Wang1, Ian A Blair1, Clementina Mesaros2
1Center of Excellence in Environmental Toxicology Center, Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia,, USA.
Abstract:
The advancement of mass spectrometry-based analytical platforms largely facilitates small-molecule metabolomics studies, which allows analysis of a large number of metabolites from bio-samples simultaneously and gives a general picture of metabolic changes related to diseases or environmental alteration. Due to the large diversity of cellular metabolites, globally and precisely examining metabolic profile remains the most challenging part in metabolomic experiment. Mass spectrometry coupled with liquid chromatography enhances the sensitivity and resolving power of metabolites identification and quantification, as well as the versatility of analyzing a wide array of metabolites. In this chapter, we discussed the technical aspects of each step in the workflow of metabolomics studies we aimed to give technical guidelines for metabolomics investigation design and approach.
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