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Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
Transcriptome profiling of embryonic development rate in rainbow trout advanced backcross introgression lines
Peng Xu1, Lauren M McIntyre, Julie Scardina
1Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
Marine Biotechnology (New York, N.Y.)
|March 31, 2010
Summary
Fast-developing rainbow trout embryos show higher expression of genes related to growth and protein synthesis. These key developmental processes begin earlier in embryos with fast-developing alleles, impacting early growth and survival.
Area of Science:
- Aquaculture and Aquatic Genomics
- Developmental Biology
- Quantitative Genetics
Background:
- Embryonic development rate in fish, like rainbow trout (Oncorhynchus mykiss), is crucial for survival and later physiological performance.
- Understanding genetic regulation of development rate can inform selective breeding and aquaculture practices.
Purpose of the Study:
- To identify genes differentially regulated between fast and slow-developing rainbow trout embryos.
- To investigate the genetic basis of a major quantitative trait loci (QTL) for embryonic development rate.
Main Methods:
- Whole genome expression microarray analysis was performed on rainbow trout embryos at 15, 19, and 28 days post-fertilization.
- Embryos from a fourth-generation backcross family were used, segregating for alleles linked to a major embryonic development rate QTL.
- Gene expression data was analyzed in relation to QTL genotype, sex, and developmental stage.
Main Results:
- 183 features showed significant differential expression between fast and slow development genotypes.
- Genes involved in cell cycle, muscle contraction, and protein synthesis were upregulated in fast-developing embryos.
- Early onset of these growth-related processes was observed in fast-developing embryos as early as 15 days post-fertilization.
Conclusions:
- Upregulation of growth and protein synthesis genes in fast-developing embryos suggests a mechanism for accelerated early development and body mass construction.
- These findings provide insights into the genetic architecture underlying embryonic development rate in rainbow trout.
- Candidate genes were primarily located outside the major embryonic development rate QTL, with one exception.
