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Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
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Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

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Nanoscale sensor design via in situ labeling of gold nanoparticles onto protein scaffolds

Zahra Afrasiabi1, Ravi Shukla, Nripen Chanda

  • 1Department of Life and Physical Sciences, Lincoln University, Jefferson City, MO 65101, USA.

Journal of Nanoscience and Nanotechnology
|April 1, 2010
PubMed

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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.